dre-mir-454a: Dre-mir-454a is a microRNA (miRNA) in zebrafish that has been evaluated for its stability as a reference gene in gene expression studies [PMC8613261]. According to the Normfinder software analysis, dre-mir-454a ranked as the third most stable miRNA with a stability value of 0.014, suggesting its potential utility as an endogenous control [PMC8613261]. However, when expression data from methimazole (MTZ) treatments were considered, dre-mir-454a's stability ranking dropped slightly to the seventh position with a value of 0.018 [PMC8613261]. Additionally, when comparing across three different zebrafish strains, dre-mir-454a's ranking was fourth in terms of stability with a value of 0.026 [PMC8613261]. The study investigated nine miRNAs including dre-mir-454a for their suitability as endogenous reference genes, indicating the importance of selecting stable reference genes for accurate normalization in gene expression analysis [PMC8613261].