bmo-mir-2845: Bmo-mir-2845 is a microRNA that has been studied for its regulatory effects on BmFib-L, a silk protein gene in Bombyx mori. Specific primers were designed to amplify BmFib-L, and its expression levels were detected using qPCR [PMC8675719]. Through gene sequence alignment and RNAhybrid analysis, it was confirmed that bmo-mir-2845 significantly inhibited the expression of BmFib-L at the cellular and individual levels [PMC8675719]. The folding free energy of bmo-mir-2845 was found to be < -20.0 kcal/mol, indicating its potential regulatory effects on BmFib-L [PMC8675719]. The regulatory capabilities of bmo-mir-2845 on BmFib-L were validated in BmN cells, and it was found that bmo-mir-2845 downregulated the expression of BmFib-L in these cells [PMC8675719]. The expression of bmo-mir-2845 increased from the 1st to 5th day in the 5th instar of Bombyx mori, while the expression of BmFib-L increased sharply [PMC8675719]. The binding site prediction and mutational analysis confirmed that bmo-mir-2845 has a regulatory role on the binding site within the 3' UTR region of BmFib-L [PMC8675719]. Furthermore, it was observed that miR-2845's regulatory function on BmFib-L showed consistent trends in both BmN cells and individuals, providing additional evidence for its downregulation effect on BmFib-L expression [PMC8675719]. Further studies are needed to fully understand the function and potential target genes regulated by bmo-mir-2845 [PMC8675719].