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rno-mir-22: rno-mir-22, a precursor DNA molecule, was synthesized by Genechem, Co., Ltd., in China [PMC5103773]. Plasmid vectors containing different forms of rno-mir-22 were obtained from GeneChem Technology Co., Ltd. [PMC6109564]. The Redd1 gene, which has a binding site for miR-22, was synthesized by GenePharma [PMC6405932]. The target mRNAs of rno-mir-22 were predicted using various databases, and Redd1 was found to directly associate with rno-mir-22 [PMC6405932]. In injured animals, rno-mir-22 and two other miRNAs were found to be down-regulated in the heart but upregulated in the kidney and lung [PMC6377737]. Primary cultures of cortical neurons and striatal neurons were infected with a lentiviral vector expressing rno-mir-22 at a specific concentration [PMC3547907]. The gene sequence for rno-mir-22 was cloned using specific primers [PMC3547907]. Lentiviral vectors encoding different genes, including the rat miR-22 gene (rno-mir-22), were produced using a four-plasmid system in HEK293T cells [PMC3547907].
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